Coding

Part:BBa_K3003023:Design

Designed by: Merve Nida Baştürk   Group: iGEM19_Bilkent-UNAMBG   (2019-10-16)


Wild-Type Insulin with a Linker Peptide


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 115
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 115
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 103
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 115
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 115
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed specifically considering the compatibility of chains with the linker sequences.


Source

Synthesized by IDT.

References

1. Chance, R. E., Dimarchi, R. D., Hoffmann, J. A., Long, H. B., & Miller, A. R. (n.d.). 2. Fu, Z., R. Gilbert, E., & Liu, D. (2012). Regulation of Insulin Synthesis and Secretion and Pancreatic Beta-Cell Dysfunction in Diabetes. Current Diabetes Reviews, 9(1), 25–53.